![]() WRKY23 expression is affected by two well-known root stem cell specification mechanisms, that is, SHORTROOT and MONOPTEROS–BODENLOS signalling and can partially rescue the root-forming inability of mp embryos. Knocking down WRKY23 transcript levels or expression of a dominant-negative WRKY23 version via a translational fusion with the SRDX repressor domain affected both apical–basal axis formation as well as installation of the root stem cell niche. The auxin-responsive AtWRKY23 transcription factor is expressed from early embryogenesis onwards and the timing and localization of its expression overlaps with the root stem cell niche. In higher plants, apical–basal patterning mechanisms are crucial to correctly specify root and shoot stem cell niches that will sustain and drive post-embryonic plant growth and development. The development of a multicellular embryo from a single zygote is a complex and highly organized process that is far from understood. ‡ These authors contributed equally to this work.6 Department Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.5 Plant Developmental Biology Wageningen University, 6700 AP Wageningen, The Netherlands.4 Laboratory of Biochemistry, Wageningen University, 6703 HA Wageningen, The Netherlands.3 Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, LE12 5RD Loughborough, UK.2 Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.1 Department of Plant Systems Biology, VIB, and Bioinformatics, Ghent University, 9052 Ghent, Belgium. ![]()
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